Interleukin-1 stimulation of human endothelial cells to produce granulocyte - macrophage colony-stimulating factor, granulocyte

Endothelial cells is an effective water-soluble products of monocyte-derived hematopoietic growth factor stimulation. Interleukin-1 (IL-1) is released by activated monocytes mediate inflammatory response. We determine whether purified recombinant human interleukin-1 stimulates human umbilical vein endothelial cells to release hematopoietic growth factors. As little as 1 U / ml IL1 growth factor to stimulate growth factor production by the endothelial cells, and the increased amount of IL-1 enhanced the production in a dose-dependent manner. The increased growth factor production continues to rise more than 48 hours within 2-4 hours. To investigate the molecular basis of these findings, oligonucleotide probes, granulocyte - macrophage colony-stimulating factor, macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) granulocyte-macrophage colony stimulating factor (M-CSF), and multi-CSF hybridization of poly (A) containing from unstimulated RNA prepared and IL1 stimulation of endothelial cells. GM-CSF, G-CSF levels significantly, but not the M-CSF or multi-CSF in detected in the mRNA of IL-1 stimulation of endothelial cells. Confirmed the presence of two kinds of GM-and the G-CSF bioassay performed on IL-1 stimulation of endothelial cells in the conditioned medium. These results indicate that the process of recombinant human IL-1 can stimulate GM-CSF and G-CSF in the release of endothelial cells, and to provide a mechanism and through this mechanism, IL-1, the inflammatory reaction in the process of two grains can be adjusted in the production and function.