Elimination of platelet factor 4 (PF4) from platelets reduces atherosclerosis in C57Bl/6

The activated platelet, the release of platelet factor 4 (PF4) is present in patients with atherosclerosis. So far, there is no direct invivo evidence exists PF4 participation in atherosclerosis. In the current study, we tested the hypothesis that, PF4 atherosclerosis the PF4 protection, genetic eliminate mice from atherosclerosis.We bred PF4-/ - mice to both atherosclerosis-like sensitive background WT C57BL / 6 (WT) and apolipoprotein E-/ - atherosclerosis importance of research, PF4. In order to promote atherosclerosis, WT and PF4-/ - mice were fed a high-fat diet 30 weeks, 10 weeks, apolipoprotein E-/ - and apoE-/ - PF4-/ - mice fed a high-fat Western-style diet. PF4-/ - exam lesions aortic root atherosclerosis diet fed mice demonstrated to reduce atherosclerosis (20% compared to WT) apoE-/ - mice showed similar changes in ApoE-/ - PF4-/ - mice showed examinations aortic atherosclerosis hardening of the burden of 37% compared apolipoprotein E-/ - mice.Although we found a similar total and non-of HDL cholesterol inWT of PF4-/ - mice levels, increased high-density lipoprotein cholesterol levels PF4-/ - in two different backgrounds. These data indicate that, for the first time, the platelet-specific chemotactic factor PF4 to promote the development of atherosclerotic lesions in vivo.