Yeast Two-hybrid screening

Two hybrid screening ( also known as the yeast two-hybrid system or precipitation ) is a molecular biology technique for the discovery of protein - protein interactions and protein RNA interaction - Test physical interactions (such as binding between two proteins ) or a single protein and nucleic acid molecules, respectively.

The premise behind the test is the activation of downstream gene (s ) on the binding of transcription factors in an upstream activating sequence ( UA ). Two-hybrid screening, transcription factor is divided into 2 segments, called the binding domain ( BD ) and activation domain ( AD ). BD is domains responsible for binding agents and advertisement domain is responsible for activation of transcription. [1] [2] of the precipitation is a protein fragment complementation assay.

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BD is domains responsible for binding agents and advertisement domain is responsible for activation of transcription.

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